THE QUANTITATIVE EXTRACTION AND PARTIAL PURIFICATION OF STEROIDS IN TISSUE AND BLOOD*

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Abstract
A method for the quantitative extraction and partial purification of steroids in tissue and blood is described. The technique consists of extraction with 4:1 methylal-methanol, solvent partition (in certain cases only), and purification by means of a simple silica-gel column. Completeness of extraction was studied by means of radioactive steroids added to, perfused through, or incubated with tissues. Silica-gel purification results in recovery of 95% or better of the steroids, with recovery of only 5 to 7% of the original weight of the crude lipid extract.