Characteristics of a Hamster Cell Clone Transformed by Polyoma Virus2
- 1 April 1963
- journal article
- Published by Oxford University Press (OUP) in JNCI Journal of the National Cancer Institute
- Vol. 30 (4) , 795-815
- https://doi.org/10.1093/jnci/30.4.795
Abstract
A cloned line of Syrian hamster kidney cells (C13) was infected with polyoma virus and clone C13-TC6 was prepared from one of the resulting transformed cells. A comparison between the transformed clone and uninfected C13 cells revealed that the following characteristics were acquired by the transformed cells: (a) a higher plating efficiency; (b) a higher production of nonvolatile acid in culture, possibly indicative of enhanced glycolytic activity; (c) an ability to initiate tumors in hamsters when 106 or more cells were inoculated subcutaneously; and (d) a tendency to accumulate chromosome abnormalities and tetraploid cells, though these abnormalities were not immediately associated with transformation. Both the C13-TC6 line and the uninfected control line C13 produced tumors when injected into the cheek pouch of the hamster.—J Nat Cancer Inst 30: 795–815, 1963.Keywords
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