Selection of a Suitable Extraction Method for Mutagenic Activity from Woodsmoke-Impacted Air Particles

1 October 1988

journal article
research article
Published by Taylor & Francis in International Journal of Environmental Analytical Chemistry

Vol. 34 (2) , 137-154
https://doi.org/10.1080/03067318808027411

Abstract

Extraction methods were evaluated for recovery of mutagenic activity from woodsmoke-impacted air particles. Soxhlet and sonication techniques were utilized with a variety of solvents to ascertain the effect of solvent choice, extraction methods, or dissolved gases in extraction solvents on the recovery of mutagenicity. Sonication extraction gave slightly less mass recovery than the Soxhlet method. Methanol extracted more mass than the other solvents with dichloromethane recovering the least. Dissolved gases were not found to have any effect, while mutagenicity was shown to be dependent upon solvent and extraction method. Soxhlet extraction with acetone and toluene/ethanol yielded the highest recovery of mutagenic activity, however, results indicated a solvent/solute interaction which chemically altered one or

Keywords

CHEMICAL ANALYSIS

This publication has 10 references indexed in Scilit:

Preparation of uniform glass fiber Hi-Vol filter sections using a stainless steel and aluminum cutting jig
Atmospheric Environment (1967), 1986
Evaluation of techniques used in the preparation of diesel extract samples for mutagenicity studies
Environment International, 1986
Mutagenicity studies of ambient airborne particles
Mutation Research/Genetic Toxicology, 1983
Mutagenicity studies of ambient airborne particles
Mutation Research/Genetic Toxicology, 1983
Comparative extraction of genotoxic components of air particulates with several solvent systems
Mutation Research Letters, 1982
An empirical approach to the statistical analysis of mutagenesis data from the Salmonella test
Mutation Research/Environmental Mutagenesis and Related Subjects, 1982
Mutagenic and carcinogenic potency of diesel and related environmental emissions: Salmonella bioassay
Environment International, 1981
Evaluation of Collection and Extraction Methods for Mutagenesis Studies on Ambient Air Particulate
Published by Springer Nature ,1980
Airborne Mutagens Bioassayed in Salmonella typhimurium2
JNCI Journal of the National Cancer Institute, 1977
Significance tests for multiple comparison of proportions, variances, and other statistics.
Psychological Bulletin, 1960