Effects of ammoniation on the ‘carry‐over’ of aflatoxins into bovine milk

Publisher Website
Google Scholar
Abstract
Two experiments were performed using lactating cows fed various treated and non‐treated commodities from AFB1 contaminated peanut cakes. Treatment with ammonia gas by an autoclaving process was used for detoxification. Two methods were used for AFMi determination in every milk sample: a TLC procedure recognized by AOAC and IDF and an HPLC method with a detection limit of 0.100 and 0.010 μg/1 respectively. In a first experiment, lactating cows were fed treated and untreated meals during periods separated by uncontaminated soya meals phases. The total excreted AFM1 was 2.6% of the total ingested AFB1 from untreated feed contaminated at 1100 μg/kg. During periods receiving treated meals in the diet, AFMI contents in milk were below 0.1 μg/I. However, by using AFM1 data obtained using the HPLC method, an AFM1/AFB1 ratio of 4.6% was found from treated feed contaminated at 40 μg AFB1/kg. In a second experiment, a herd of 50 lactating cows was used for a long term (16 months) feeding of mixed commodities containing 30% ammoniated peanut cakes. AFB1 residues in the treated diet were below 10 μg/kg, the EEC action level, and no AFM1 residue was found up to 0.1 μg/1 in collected milk throughout this experiment.