The GMD1 and GMD2 Genes of Arabidopsis Encode Isoforms of GDP-D-Mannose 4,6-Dehydratase with Cell Type-Specific Expression Patterns
Open Access
- 1 June 2003
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 132 (2) , 883-892
- https://doi.org/10.1104/pp.103.022368
Abstract
L-Fucose (l-Fuc) is a monosaccharide constituent of plant cell wall polysaccharides and glycoproteins. The committing step in the de novo synthesis of l-Fuc is catalyzed by GDP-d-mannose 4,6-dehydratase, which, in Arabidopsis, is encoded by the GMD1 and GMD2 (MUR1) genes. To determine the functional significance of this genetic redundancy, the expression patterns of both genes were investigated via promoter-β-glucuronidase fusions and immunolocalization of a Fuc-containing epitope. GMD2 is expressed in most cell types of the root, with the notable exception of the root tip where strong expression of GMD1 is observed. Within shoot organs, GMD1::GUS expression is confined to stipules and pollen grains leading to fucosylation of the walls of these cell types in the mur1 mutant. These results suggest that GMD2 represents the major housekeeping gene for the de novo synthesis of GDP-l-Fuc, whereas GMD1 expression is limited to a number of specialized cell types. We conclude that the synthesis of GDP-l-Fuc is controlled in a cell-autonomous manner by differential expression of two isoforms of the same enzyme.Keywords
This publication has 23 references indexed in Scilit:
- Distribution of Fucose-Containing Xyloglucans in Cell Walls of the mur1 Mutant of ArabidopsisPlant Physiology, 2003
- Requirement of Borate Cross-Linking of Cell Wall Rhamnogalacturonan II for Arabidopsis GrowthScience, 2001
- Synthesis of GDP-L-fucose by the Human FX ProteinJournal of Biological Chemistry, 1996
- Generation of Monoclonal Antibodies against Plant Cell-Wall Polysaccharides (I. Characterization of a Monoclonal Antibody to a Terminal [alpha]-(1->2)-Linked Fucosyl-Containing EpitopePlant Physiology, 1994
- Altered Growth and Cell Walls in a Fucose-Deficient Mutant of ArabidopsisScience, 1993
- Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the walls during growthThe Plant Journal, 1993
- Structure and Function of Plant Cell WallsPublished by Elsevier ,1988
- Genomic sequencing.Proceedings of the National Academy of Sciences, 1984
- Secretory Activity of the Root CapPublished by Springer Nature ,1981
- A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue CulturesPhysiologia Plantarum, 1962