Stimulative Effects of Chelating Agents, 2, 2’-Bipyridine and 1, 10-Phenanthroline, on Lipid Peroxidation in Rat Liver Microsomes

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Abstract
Well known lipid peroxidation inhibitors, 1,10-phenanthroline and 2,2''-bipyridine, stimulated microsomal NADPH- and ascorbic acid-dependent lipid peroxidation when low concentrations of these chelating agents were added to incubation mixture. The stimulatory effects of the chelating agents on lipid peroxidation were enhanced when Fe2+ was added together with the chelating agents to the mixture at a molar ratio of 1:1. EDTA had no stimulatory effect on lipid peroxidation. Fe2+ EDTA complex increased lipid peroxidation by only 20-30% which was lower than that obtained by the addition of the same concentration of Fe2+ alone. Mn2+ and Ca2+ which are also inhibitors of lipid peroxidation, had no ability to stimulate lipid peroxidation even in the presence of extra Fe2+. Changes in the lipid peroxidation by chelating agents affected the apparent activity of ethylmorphine N-demethylation.