The Importance of PAPS in Determining Sulfation in Gastrointestinal Mucosa
- 31 December 1975
- journal article
- research article
- Published by S. Karger AG in Digestion
- Vol. 14 (4) , 372-375
- https://doi.org/10.1159/000197955
Abstract
The in vitro sulfation of glycoproteins, mucopolysaccharides and lipids was studied with rat stomach, duodenum and colon mucosal scrapings. In the presence of Na235SO4, the incorporation of label was colon > stomach ≥ duodenum. In the presence of [35S] 3’-phosphoadenosine 5’-phosphosulfate (PAP35S), the incorporation of label was colon = stomach > duodenum. The 35S was incorporated into glycoprotein, mucopolysaccharide and sulfatide. It is suggested that the availability of PAPS may be an important factor in determining the differences in sulfation previously observed histochemically in several species, namely that in vivo colonic mucosa far exceeds stomach mucosa in synthesis of sulfated polyanions.Keywords
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