Effects of Cytosine Methylation on Pyrimidine Dimer Formation in DNA
- 1 January 2000
- journal article
- Published by Wiley in Photochemistry and Photobiology
- Vol. 71 (2) , 162-5
- https://doi.org/10.1562/0031-8655(2000)071<0162:eocmop>2.0.co;2
Abstract
The relative induction of cyclobutane pyrimidine dimers (CPD) and pyrimidine (6-4)pyrimidone photoproducts ([6-4]PD) was quantified in the duplex homopolymers polydeoxyadenosine:polydeoxythymidine, polydeoxyguanosine:polydeoxycytidine and polydeoxyguanosine:polydeoxy-5-methylcytidine irradiated with UVC or UVB radiation. Cytosine methylation significantly increased the yield of cytosine (6-4)PD after irradiation with UVC light and of cytosine CPD and (6-4)PD after irradiation with UVB light. The data suggest that CPD and (6-4)PD are preferentially induced at 5-methylcytosine bases in DNA of cells exposed to sunlight and comprise a major component of the mutation spectrum leading to the initiation of sunlight-induced skin cancer.Keywords
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