Dextran Sulfate Plaque Variants of Attenuated Type 1 Poliovirus

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Abstract
Summary: Analysis of a stock of the NIH reference attenuated, type 1 poliovirus, NA-2 (Sabin strain) by a previously described procedure employing dextran sulfate (dex-s) in the overlay medium, showed that it consisted of three different plaque types. Two of the enhanced (10 mm) and two of the inhibited (less than 1 mm) plaque types were examined for their behavior in tests using the d and rct/40 markers. The enhanced or ds+ virus was found to be d+, rct/40-, whereas the inhibited or ds- virus was d-, rct/40-. One of the ds+ viruses produced histologic lesions of poliomyelitis but no paralysis in a high proportion (55%) of the monkeys after intrathalamic inoculation, whereas the other produced no lesions; the two ds- types caused minimal or no lesions.

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