Corn Cystatin I Expressed in Escherichia coli: Investigation of Its Inhibitory Profile and Occurrence in Corn Kernels
- 1 September 1994
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 116 (3) , 488-492
- https://doi.org/10.1093/oxfordjournals.jbchem.a124551
Abstract
Corn cystatin I was expressed in Escherichia coli as a mature protein. It was purified by gel filtration on Sephadex G-50, ion-exchange HPLC, and reversed-phase HPLC. The purified protein showed strong inhibitory activities against papain (Ki: 3.7×10–8M), and cathepsins H (Ki: 5.7×10−9M) and L (Ki: 1.7×10−8 M), whereas it inhibited cathepsin B to a lesser extent (Ki: 2.9 × 10−7 M). Western blot analysis using antibody raised against corn cystatin I revealed that in the corn kernel, the protein occurs with a molecular mass of approximately 13 kDa. Localization in the aleurone layer and embryo of the corn kernel was shown by immunostaining microscopy.Keywords
This publication has 0 references indexed in Scilit: