The utilization of itaconate by Pseudomonas sp

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Abstract
Washed suspension of Pseudomonas B2aba grown on itaconate oxidized itaconate, DL-citramalate and mesaconate; but these substances were not oxidized by succinate-grown cells. Extracts of the itaconate-grown organism catalyzed the formation of pyruvate and acetyl-coenzyme-A when incubated with itaconate, adenosine-triphosphate (ATP), coenzyme-A and Mg2+ ions. The quantities of pyruvate formed were proportional to those of coenzyme-A added. Synthetic itaconyl-coenzyme-A replaced the requirement for itaconate, ATP and coenzyme-A. The formation of pyruvate and acetyl-coenzyme-A from DL-citramalate, in the presence of extract and Mg2+ ions, necessitated the presence either of succinate, coenzyme-A ATP, or succinyl-coenzyme-A. Both synthetic itaconyl-coenzyme-A and enzymically produced citramalyl-coenzyme-A yielded pyruvate and acetyl-coenzyme-A in equimolar amounts. The enzymes involved in these reactions were isolated and identified. Their inducibility is important in the utilization of itaconate.