Effect of Bilirubin on the Arrhenius Plots for Na, K‐ATPase Activities of Young and Adult Rat Cerebra
- 1 March 1981
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 36 (3) , 826-829
- https://doi.org/10.1111/j.1471-4159.1981.tb01668.x
Abstract
The effect of bilirubin on the temperature dependence of Na, K-ATPase activity was investigated with NaI-treated microsomes prepared from young (13- to 23-day-old) and adult (about 1-year-old) rat cerebra. The Arrhenius plots for the enzymic activities in the young and adult rats showed break temperatures at 26 and 18°C, respectively. In the young rat enzyme, bilirubin caused a shift of the break temperature to 23 and 22°C at concentrations of 60 and 120 μM, respectively, with a significant increase of the activation energy below the break temperature. However, no significant changes in the break temperature and the activation energy were observed in the adult rat enzyme exposed to the same concentrations of the pigment. The results suggest that the lipid environment surrounding the enzyme and modulating its activity in the plasma membranes may differ between the young and adult rat cerebra, and that bilirubin may change the physical state of the lipids related to the activity of the young rat Na, K-ATPase.Keywords
This publication has 20 references indexed in Scilit:
- Rate limitation of (Na+ + K+)-stimulated adenosinetriphosphatase by membrane acyl chain ordering.Proceedings of the National Academy of Sciences, 1979
- The (Na+ + K+)-activated ATPase Enzymatic and transport propertiesBiochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics, 1979
- Interactions of small molecules with phospholipid bilayers. Binding to egg phosphatidylcholine of some organic anions (bromosulphophthalein, oestrone sulphate, haem and bilirubin) that bind to ligandin and aminoazo-dye-binding protein ABiochemical Journal, 1979
- USE OF ARRHENIUS PLOTS OF Na‐K ATPase and ACETYLCHOLINESTERASE AS A TOOL FOR STUDYING CHANGES IN LIPID‐PROTEIN INTERACTIONS IN NEURONAL MEMBRANES DURING BRAIN DEVELOPMENTJournal of Neurochemistry, 1979
- Regulation of membrane enzymes by lipidsBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1978
- Changes in (sodium + potassium ion)-dependent ATPase activity of Ehrlich ascites tumor cells produced by alteration of membrane fatty acid compositionBiochemistry, 1976
- Lipid dependence of activity-temperature relationship of (Na+, K+)-activated ATPaseBiochimica et Biophysica Acta (BBA) - Biomembranes, 1973
- Role of lipid-phase transitions in the regulation of the (sodium + potassium ion)-dependent adenosinetriphosphataseBiochemistry, 1973
- THE CHANGE IN FATTY ACID COMPOSITION OF RAT BRAIN PHOSPHOLIPIDS DURING DEVELOPMENTJournal of Neurochemistry, 1966
- Stimulation and solubilization of the sodium ion-activated adenosine triphosphatase of cerebral microsomes by surface-active agents, especially polyoxyethylene ethers: actions of phospholipases and a neuraminidaseBiochemical Journal, 1964