Synthesis and secretion of proteins resembling platelet-derived growth factor by human glioblastoma and fibrosarcoma cells in culture.

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Abstract
Immunoprecipitation of protein extracted from metabolically labeled human glioblastoma and fibrosarcoma cells with antiserum to platelet-derived growth factor (PDGF) showed that these cells express and secrete proteins that are recognized specifically by the antiserum. The MW of immunoprecipitated proteins in the lysates of the malignant cells ranged from 16 [kilodalton]-140 kDa [kilodalton]. Both cell lines secreted a 31-kDa polypeptide with structural, immunological and biological properties similar to those of human PDGF. These cell lines synthesized a 4.4-kb [kilobase] mRNA that contained sequences from all the 6 currently identified exons of the human c-sis gene. The PDGF-like proteins in the 2 mesenchyme-derived transformed cells are encoded at least in part by the c-sis locus.