Kinetic analysis of a graft reaction induced in cell culture.

  • 1 November 1969
    • journal article
    • Vol. 17  (5) , 723-40
Abstract
A kinetic analysis was made of the graft reaction induced in culture by plating rat lymphocytes on monolayers of mouse fibroblasts. The immune cytolysis of the mouse fibroblasts, carried out by sensitized rat large pyroninophilic cells (LPC), was determined by the rate of 51Cr released from labelled target cells. The sensitivity and accuracy of the quantitative method employed in the present study was evident from: (a) the low spontaneous release of the 51Cr from the labelled fibroblasts, (b) the lack of re-utilizaiton of the released 51Cr by lymphocytes, and (c) the low standard deviation of replicate plates.