The peroxisomal targeting signal of 3‐oxoacyl‐coA thiolase from Saccharomyces cerevisiae

Publisher Website
Google Scholar
Abstract
All peroxisomal 3‐oxoacyl‐CoA thiolases identified so far do not contain the previously identified tripeptide peroxisomal targeting signal at their carboxy‐termini. For the two rat thiolases it was shown that their peroxisomal targeting signals are localized within the amino‐terminal region of the proteins and are cleaved upon import. This report demonstrates that the N‐terminal region of the peroxisomal 3‐oxoacyl‐CoA thiolase from Saccharomyces cerevisiae is essential for its peroxisomal targeting, and that the N‐terminal 16 amino acids of yeast thiolase are sufficient to target the otherwise cytosolic small subunit of ribulose‐1,5‐bisphosphate carboxylase to peroxisomes for import.