P1,P4‐diadenosine 5′ tetraphosphate induces nitric oxide release from bovine aortic endothelial cells
- 15 May 1998
- journal article
- Published by Wiley in FEBS Letters
- Vol. 427 (3) , 320-324
- https://doi.org/10.1016/s0014-5793(98)00454-2
Abstract
Since the infusion of P1,P4‐diadenosine 5′ tetraphosphate (Ap4A) into animal models induces vasodilation [1, 2], the present study was performed to determine whether Ap4A induces the release of nitric oxide (NO) from endothelial cells. Ap4A induced NO release was 4.2‐fold greater than the amount of NO released under basal condition. Ap4A induced NO release was inhibited by N G‐nitro‐l‐arginine (L‐NNA) and this inhibition was reversed by L‐Arg. In addition, EGTA inhibits Ap4A induced NO release. These data are consistent with Ap4A inducing the release of NO from endothelial cells through the activation of endothelial nitric oxide synthase.Keywords
This publication has 35 references indexed in Scilit:
- Cardiovascular effects and influence on blood gasesActa Anaesthesiologica Scandinavica, 1994
- The adenosine 5',5''',P1,P4-tetraphosphate receptor is at the cell surface of heart cellsBiochemistry, 1993
- Continuous Monitoring of Nitric Oxide Release from Human Umbilical Vein Endothelial CellsBiochemical and Biophysical Research Communications, 1993
- Adenine dinucleotide-mediated activation of glycogen phosphorylase in isolated liver cellsCellular Signalling, 1993
- Presence of diadenosine polyphosphates— Ap4A and Ap5A— In rat brain synaptic terminals. Ca2+ dependent release evoked by 4-aminopyridine and veratridineNeuroscience Letters, 1992
- L-arginine is the physiological precursor for the formation of nitric oxide in endothelium-dependent relaxationBiochemical and Biophysical Research Communications, 1988
- Vascular endothelial cells synthesize nitric oxide from L-arginineNature, 1988
- Catabolism of Ap4A and Ap3A in whole bloodEuropean Journal of Biochemistry, 1988
- Exogenous ATP raises cytoplasmic free calcium in fura‐2 loaded piglet aortic endothelial cellsFEBS Letters, 1986
- Characterization of a homogeneous complex of arginyl- and lysyl-tRNA synthetase: zinc and adenosine 5'-phosphate dependent synthesis of diadenosine 5',5'''-P1,P4-tetraphosphateBiochemistry, 1983