Highly Sensitive Immunoassays for Three Forms of Rat Brain Enolase

Publisher Website
Google Scholar
Abstract
Highly sensitive enzyme irnmunoassay systems for three forms (αα, αγ, and γγ) of rat brain enolase were prepared by use of specific antisera to two distinct subunits (α and γ) of the isozymes and β‐D‐galactosidase from Escherichia coli as label. Less than fmol‐levels of the homologous dimer forms (αα and γγ) could be determined with the corresponding antibody F(ab′)2‐bound solid‐phase and the antibody Fab′‐βD‐galactosidase complex. The hybrid form (αγ) could also be assayed specifically by use of the antibody to one subunit as solid‐phase, and the antibody to another subunit as labelled complex with a minimum detectable sensitivity of 1 fmol.