Detection of the ras p21 gene product in human leukemias by flow cytometry
- 1 January 1989
- journal article
- research article
- Published by Wiley in Journal of Clinical Laboratory Analysis
- Vol. 3 (2) , 108-115
- https://doi.org/10.1002/jcla.1860030208
Abstract
To date, the detection of oncogene products in human neoplasms has relied primarily upon immunoblot analysis of specimen homogenates. Herein are reported the results of a study using flow cytometry to evaluate the expression of the ras p21 gene product in both fresh and cryopreserved specimens of human acute leukemias. Cell lines known to express ras p21 were used as positive controls and normal peripheral blood was used as a negative control. Intensity of staining for ras p21 (lp21) was expressed as the ratio of the peak channel numbers of the peak generated by staining with anti‐ras p21 to the peak obtained by staining with an isotype control. Using this method, 21 out of 32 clinical specimens of acute leukemia were found to express ras p21 in elevated amounts compared to normal peripheral blood. Flow cytometry appears to be a practical method for routine screening of clinical specimens for the expression of oncogene products on individual cells rather than cell homogenates.Keywords
This publication has 21 references indexed in Scilit:
- Relationship between an activated N-ras oncogene and chromosomal abnormality during leukemic progression from myelodysplastic syndromeBlood, 1988
- Ras Oncogene p21 Levels Parallel Malignant Potential of Different Human Colonic Benign ConditionsArchives of Surgery, 1987
- Activation mechanism of the N-ras oncogene in human leukemias detected by synthetic oligonucleotide probesBiochemical and Biophysical Research Communications, 1987
- Mutations in N-ras predominate in acute myeloid leukemiaBlood, 1987
- Detection of an intracellular transforming protein (v-Ki-ras p21) using the flow activated cell sorter (FACS)In Vitro Cellular & Developmental Biology - Plant, 1986
- Detection of the intracellular ras p21 oncogene product by flow cytometryAnalytical Biochemistry, 1986
- High frequency of N-ras activation in acute myelogenous leukemiaBlood, 1986
- Cellular ras oncogene expression and cell cycle measured by flow cytometry in hematopoietic cell linesBlood, 1986
- Activation of N-ras gene in bone marrow cells from a patient with acute myeloblastic leukaemiaNature, 1984