An Application of the Prussian Blue Technique to a Light Microscope Study of Water Movement in Transpiring Leaves of Cotton (Gossypium hirsutumL.)

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Abstract
Prussian blue deposition in transpiring leaves of Gossypium hirsutum L. indicated that water moved rapidly from the tracheary elements by a path along the bundle sheath parenchyma to the bundle sheath extension parenchyma and then laterally through the epidermis to the sub-stomatal cavities or to the tnchomes. A slower pathway of water movement to the substomatal cavities occurred through the mesophyll. The path of Prussian blue deposition indicated that waterleft the leaf through the stomata and to a lesser extent through the trichomes. In the outer periclinal wall of epidermalcells Prussian blue was deposited below but not within the cuticle.

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