Cephabacins, new cephem antibiotics of bacterial origin. II. Isolation and characterization.

Abstract
Fifteen components of new antibiotics, cephabacins, were isolated from the culture filtrates of Lysobacter lactamgenus YK-90, Xanthomonas lactamgena YK-280 and X. lactamgena YK-278. They were purified by column chromatography using cation-exchange resins, activated C, high porous resins and cation-exchange Sephadex and by preparative reverse-phase high performance liquid chromatography. The basic, water-soluble antibiotics were characterized as having a cephem skeleton and oligopeptide(s) as a side chain constituent from their spectroscopic analyses and amino acid analyses.