Relationships between nucleoside triphosphate pyrophosphohydrolase activity and inosine triphosphate accumulation in human erythrocytes
- 1 October 1976
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 54 (10) , 843-847
- https://doi.org/10.1139/o76-121
Abstract
The relationship between nucleoside triphosphate pyrophosphophydrolase (NTPH) (EC 3.6.1.19) activity in erythrocyte lysates and accumulation of radioactive ITP in human erythrocytes incubated in vitro with [14C]hypoxanthine, was studied in 93 humans. When ITP accumulation, expressed as percentage of total radioactive nucleotides, was plotted against NTPH specific activity, an inverse relationship existed. A continuous spectrum of NTPH specific activities and ITP accumulation values existed in the human population and the relationship between these 2 parameters followed the relationship of substrate concentration to enzyme activity predicted by Michaelis-Menten enzyme kinetics. One interpretation of these data is that the ITP concentration in human red blood cells is controlled by the degradation of ITP to IMP and pyrophosphate catalzyed by NTPH.This publication has 1 reference indexed in Scilit:
- Statistical estimations in enzyme kineticsBiochemical Journal, 1961