Methylene Blue as an Inhibitor of Acetylcholin-Esterase.

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Abstract
Methylene blue (I) was a true competitive inhibitor in the acetylcholine-esterase system, and behaved similarly to other quaternary ammonium bases. The optimum acetylcholine concn. had to be higher in the presence of the dye. At 3.3 x 10-2 [image] acetylcholine. acetylcholine-esterase was inhibited 12% by 1.07 x 10-5 [image] I; at 10 times lower acetylcholine concn., the inhibition by the same dye concn. was 58%. With serum cholinesterase, these values were 89% and 98.5% respectively. The dissociation constant of the inhibitor-enzyme complex obtained under certain conditions was 5.7 x 10-7.

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