Influence of dextrose on the viability of Bacillus subtilis

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Abstract
Dextrose causes an increase in viable count of Bacillus subtilis and the effect is greater for spores than vegetative cells. The proportion of spores in which germination is initiated is not influenced by dextrose. During germination the ability of dextrose to affect the viable count is completely lost but re-appears as the germinated spore changes into a dividing vegetative cell. When spores are incubated in broth before plating in nutrient agar there is an increase in viable count followed by a fall before the increase in count due to division is detected. When plated in dextrose agar this initial rise is not seen and there is a fall in count before onset of division. The magnitude of the increase in count caused by dextrose is dependent on the method of plating. For maximum recovery of viable organisms the combination of roll-rube method with dextrose agar is to be preferred to surface plating with or without dextrose in the counting medium.

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