Incidence and Characteristics of Vancomycin Nonsusceptible Strains of Methicillin-ResistantStaphylococcus aureusat Hershey Medical Center
- 1 December 2008
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 52 (12) , 4510-4513
- https://doi.org/10.1128/aac.01073-08
Abstract
All 982 methicillin-resistantStaphylococcus aureusstrains collected from August 2006 to December 2007 were tested for vancomycin susceptibility by using 3-μg/ml vancomycin brain heart infusion screening plates, a vancomycin Etest, and a vancomycin/teicoplanin macro Etest. Three vancomycin-intermediateStaphylococcus aureus(VISA) (0.3%) and two heterogeneous VISA (0.2%) isolates were identified. The screening method yielded 895 cases of ≤1 colony and 87 positive results (with growth of >1 colony after 48 h); further Etests showed 82/87 isolates with growth on screening plates to be false positive. Repeat testing showed a false-positivity rate of only 15 of the original 87 isolates by plate screening.Keywords
This publication has 24 references indexed in Scilit:
- Characterization of Vancomycin-Heteroresistant Staphylococcus aureus from the Metropolitan Area of Detroit, Michigan, over a 22-Year Period (1986 to 2007)Journal of Clinical Microbiology, 2008
- Evaluation of a New Etest Vancomycin-Teicoplanin Strip for Detection of Glycopeptide-Intermediate Staphylococcus aureus (GISA), in Particular, Heterogeneous GISAJournal of Clinical Microbiology, 2008
- Vancomycin-Resistant Staphylococcus aureus in the United States, 2002-2006Clinical Infectious Diseases, 2008
- Reduced glycopeptide susceptibility in methicillin-resistant Staphylococcus aureus (MRSA)International Journal of Antimicrobial Agents, 2007
- The Rationale for Revising the Clinical and Laboratory Standards Institute Vancomycin Minimal Inhibitory Concentration Interpretive Criteria for Staphylococcus aureusClinical Infectious Diseases, 2007
- Prevalence and Characteristics of Heteroresistant Vancomycin-Intermediate Staphylococcus aureus Bacteremia in a Tertiary Care CenterJournal of Clinical Microbiology, 2007
- Staphylococcus aureus with Heterogeneous Resistance to Vancomycin: Epidemiology, Clinical Significance, and Critical Assessment of Diagnostic MethodsAntimicrobial Agents and Chemotherapy, 2003
- New Method for Typing Staphylococcus aureus Strains: Multiple-Locus Variable-Number Tandem Repeat Analysis of Polymorphism and Genetic Relationships of Clinical IsolatesJournal of Clinical Microbiology, 2003
- Evaluation of Current Methods for Detection of Staphylococci with Reduced Susceptibility to GlycopeptidesJournal of Clinical Microbiology, 2001
- First Report of Methicillin-Resistant Staphylococcus aureus with Reduced Susceptibility to Vancomycin in ThailandJournal of Clinical Microbiology, 2001