Variation of Estimated Numbers of Milk Somatic Cells Stained with Wright's Stain or Pyronin Y – Methyl Green Stain

Abstract
The number of somatic cells in milk samples was determined in duplicate from Breed smears stained with Wright''s and pyronin-Y-methyl-green stain. The pyronin-Y-methyl-green stain resulted in a 1/3 reduction in the error variance associated with determinations of cell numbers. Expected 95% confidence intervals indicated that about 200 smears should be made from a milk sample to obtain a 95% confidence interval as low as 50 x 104 cells/ml of milk.

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