REPLICATION OF AFRICAN SWINE FEVER VIRUS IN CELL-CULTURES

  • 1 January 1980
    • journal article
    • research article
    • Vol. 41  (9) , 1357-1367
Abstract
Infection-specific, nonstructural, African swine fever virus antigens, as visualized by the immunofluorescence technique, appeared as fine stipplings, evenly distributed in the cytoplasm and nucleus of [African green monkey kidney] Vero cells and in monocytes by postinoculation hour (PIH) 3. Cytoplasmic inclusion bodies (IB) appeared in PIH 4 and continued to increase in size up to PIH 8. The viral DNA was solely synthesized within the IB of infected monocytes, as evidenced by an autoradiographic chase experiment; maximum synthesis occurred at PIH 5. The 2300-S and 2500-S (sedimentation coefficient) structural viral antigens were visualized in the IB at PIH 5 and 6, respectively. The infective new progeny of African swine fever virus were formed between PIH 7-8, and the cell surface viral antigens were produced between PIH 8 and 9. Free virus was released between PIH 10 and 11. Infected cells detached from the glass surface from PIH 13. The 5-iodo-2''-deoxyuridine interfered with the formation of infective virus, but did not interfere with the production of viral antigens.