Chromosomal mapping ofXenopus5S genes: somatic-type versus oocyte-type
- 1 January 1983
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 11 (8) , 2313-2323
- https://doi.org/10.1093/nar/11.8.2313
Abstract
Xenopus 5S RNA genes exhibit a pattern of differential expression during development in which some members (oocyte-type) are transcribed only in oocytes, while others (somatic-type) are expressed in both oocytes and somatic cells. Using cloned DNA probes specific for each gene type, we determined the positions of these genes on Xenopus metaphase chromosomes by in situ hybridization. Somatic-type 5S genes in both X. laevis and X. borealis are located at the distal end of the long arm of only one chromosome (number 9). The oocyte-type 5S RNA genes are found at the distal ends of the long arms of most Xenopus chromosomes, including chromosome 9. Thus, large scale differences in chromosomal location cannot explain the selective expression of these genes, as suggested previously.Keywords
This publication has 25 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Oocyte extracts reactivate developmentally inert Xenopus 5S genes in somatic nucleiNature, 1982
- Transcription of Xenopus 5S ribosomal RNA genesNature, 1982
- Specific interaction of a purified transcription factor with an internal control region of 5S RNA genesCell, 1980
- The ovalbumin gene: Alleles created by mutations in the intervening sequences of the natural geneCell, 1979
- The nucleotide sequence of oocyte 5S DNA in xenopus laevis. I. The AT-rich spacerCell, 1978
- The isolation and characterization of a second oocyte 5S DNA from xenopus laevisCell, 1977
- Sequences of 5S ribosomal RNA from Xenopus mulleri and the evolution of 5S gene-coding sequencesCell, 1976
- Control of 5S RNA synthesis in Xenopus laevisNature, 1976
- 5 S DNAs of Xenopus laevis and Xenopus mulleri: Evolution of a gene familyJournal of Molecular Biology, 1973