SNAP‐25 is present in a SNARE complex in adrenal chromaffin cells
- 5 September 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 351 (2) , 207-210
- https://doi.org/10.1016/0014-5793(94)00833-7
Abstract
SNAP-25 (synaptosomal-associated protein 25 kDa) is a target for botulinum neurotoxins A and E, which both inhibit neurotransmitter release, and was recently identified together with syntaxin and synaptobrevin as receptors for NSF and α-SNAP. We show that SNAP-25 was enriched in the microsomal fraction from adrenal medulla, although the level of SNAP-25 in adrenal medullary microsomes was about 20-fold less than in brain microsomes. Immunocytochemistry confirmed the presence of SNAP-25 in cultured chromaffin cells and showed plasma membrane staining. Using immunoprecipitation, we found that SNAP-25 was present in a complex with syntaxin, synaptobrevin, synaptotagmin, NSF, α-SNAP and other unidentified polypeptides. These data indicate that SNAP-25 in chromaffin cells is present in a complex similar to that identified in brain.Keywords
This publication has 34 references indexed in Scilit:
- Synaptic vesicle fusion complex contains unc-18 homologue bound to syntaxinNature, 1993
- Botulinum neurotoxins serotypes A and E cleave SNAP‐25 at distinct COOH‐terminal peptide bondsFEBS Letters, 1993
- A complex of rab3A, SNAP‐25, VAMP/synaptobrevin‐2 and syntaxins in brain presynaptic terminalsFEBS Letters, 1993
- Tetanus and botulism neurotoxins: a new group of zinc proteasesTrends in Biochemical Sciences, 1993
- Structure of the Chicken Gene for SNAP-25 Reveals Duplicated Exons Encoding Distinct Isoforms of the ProteinJournal of Molecular Biology, 1993
- On the structure of the ‘synaptosecretosome’ Evidence for a neurexin/synaptotagmin/syntaxin/Ca2+channel complexFEBS Letters, 1993
- SNAP receptors implicated in vesicle targeting and fusionNature, 1993
- SNAPs, a family of NSF attachment proteins involved in intracellular membrane fusion in animals and yeastPublished by Elsevier ,1990
- A major role for protein kinase C in calcium‐activated exocytosis in permeabilised adrenal chromaffin cellsFEBS Letters, 1988
- Surface expression of neural cell adhesion molecule in cultured bovine paraneurones: Immunogold and immunoperoxidase methods comparedNeuroscience Letters, 1986