Extraction, Spectrophotometric, and Atomic Absorption Spectrophotometric Determination of Selenium With N-Phenylbenzohydroxamic Acid

Abstract
Solvent extraction spectrophotometric and atomic absorption spectrophotometric methods for the determination of selenium(IV) in microgram quantities are described. The selenium(IV) forms yellow colored complex with N-phenylbenzohydroxamic acid (PBHA) extractable into chloroform from 7 M HClO4. Se-PBHA complex has maximum absorbance at 345 nm with a molar absorptivity 1.5 × 105 1 mol−1 cm−1 and Sandell's sensitivity 0.000526 μg /cm2. Effect of molarity, reagent concentration, diverse ions on the extraction of selenium complex were studied. The selenium is determined in presence of tellurium.