The membrane proximal region of the cytoplasmic domain of the growth hormone receptor is involved in the activation of Stat 3
Open Access
- 7 August 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 369 (2-3) , 169-172
- https://doi.org/10.1016/0014-5793(95)00734-q
Abstract
Growth hormone receptor (GHR) signaling involves activation of the Janus Kinases (Jak) and of Stat proteins (signal transducers and activators of transcription). Growth hormone (GH) induces transcriptional activation of c‐fos gene and the c‐sis inducible element (SIE) of its promoter was shown to bind the Stat proteins. Using cells co‐transfected with GHR and Stat 3 expression vectors, we directly demonstrate that GH induces tyrosine phosphorylation of Stat 3 and its binding to the SIE probe. We showed, using mutant forms of GHR, that only the cytoplasmic membrane proximal domain of the receptor, including a conserved proline rich region (box 1), is required for this effect.Keywords
This publication has 26 references indexed in Scilit:
- In vivo growth hormone treatment rapidly stimulates the tyrosine phosphorylation and activation of Stat3Molecular Endocrinology, 1995
- Interaction of the growth hormone receptor cytoplasmic domain with the JAK2 tyrosine kinaseEndocrinology, 1994
- Signaling by the cytokine receptor superfamily just another kinase storyTrends in Endocrinology & Metabolism, 1994
- Stat3: a STAT Family Member Activated by Tyrosine Phosphorylation in Response to Epidermal Growth Factor and Interleukin-6Science, 1994
- A Single Phosphotyrosine Residue of Stat91 Required for Gene Activation by Interferon-γScience, 1993
- Identification of JAK2 as a growth hormone receptor-associated tyrosine kinaseCell, 1993
- Evidence for generation of the growth hormone-binding protein through proteolysis of the growth hormone membrane receptorEndocrinology, 1993
- Growth hormone induces c-fos and c-jun expression in cells with varying requirements for differentiationEndocrinology, 1992
- A novel family of growth factor receptors: A common binding domain in the growth hormone, prolactin, erythropoietin and IL-6 receptors, and the p75 IL-2 receptor β-chainBiochemical and Biophysical Research Communications, 1989
- Mode of Action of Pituitary Growth Hormone on Target CellsAnnual Review of Physiology, 1985