Effect of Hormone on Human Mammary Duct in Vitro
- 1 July 1978
- journal article
- research article
- Published by Georg Thieme Verlag KG in Hormone and Metabolic Research
- Vol. 10 (04) , 337-340
- https://doi.org/10.1055/s-0028-1093426
Abstract
Human mammary ducts were maintained in median-term organ culture. DNA synthesis was assessed by the uptake of 3H-thymidine in epithelial cells; labeling indices were counted on autoradiographies. DNA synthesis grossly remained between starting values in the basic conditions without hormones. Insulin (5 µg/ml) did not stimulate the uptake of the isotope as compared with the controls. Estradiol (0.001 µg/ml) and progesterone (1 µg/ml) highly significantly enhanced DNA synthesis; subsequent waves did occur.Keywords
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