Factors Affecting the Growth and Pigmentation of Cladochytrium Replicatum

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Abstract
A study of factors affecting the growth and pigmentation of Cladochytrium replicatum Karling revealed that it grew best from 15° to 25° C and in media having an initial pH of between 5.9 and 7.0. The fungus was dependent upon its environment for thiamin and the incorporation of 50 μg of that vitamin per liter of nutrient solution resulted in optimal harvests. Light was not found to influence yield or pigmentation whereas the fungus did develop coloration in those agitated media in which it grew vigorously. Under the latter conditions growth occurred in the form of orange star-shaped masses ranging from 2 to 6 mm in diameter, whereas the rhizomycelial mats formed in stationary culture did not become pigmented. C. replicatum assimilated nitrogen in the form of inorganic salts and gave optimal yields when supplied with glucose, fructose, mannose, or melibiose as carbon sources.