An immobilized hybridoma culture perfusion system for production of monoclonal antibodies
- 1 November 1988
- journal article
- Published by Springer Nature in Cytotechnology
- Vol. 1 (4) , 331-337
- https://doi.org/10.1007/bf00365078
Abstract
A fixed bed perfusion system for hybridoma cell immobilization is presented. The system consists of a culturing vessel (300 ml total volume) in which polyurethane (PU) sponges in the form of small cubes of about 5 mm sides are packed. Cells are immobilized by physical entrapment in the foam matrix. By entrapment of the cells in the pores of the matrix high cell concentration can be maintained in a mechanically protected environment. Medium is continuously circulated by an airlift pump mounted in the cell-free chamber (700 ml total volume).Keywords
This publication has 15 references indexed in Scilit:
- Immobilized cells: a review of recent literatureEnzyme and Microbial Technology, 1987
- IN VIVO MECHANISMS OF ALLOREACTIVITYTransplantation, 1986
- Ceramic-supported Hybridomas for Continuous Production of Monoclonal AntibodiesAnnals of the New York Academy of Sciences, 1983
- Entrapment of animal cells for production of monoclonal antibodies and other biomoleculesNature, 1983
- Microencapsulation of Living Cells and TissuesJournal of Pharmaceutical Sciences, 1981
- Biological particles of given size, shape, and density for use in biological reactorsBiotechnology & Bioengineering, 1979
- SPONGE MATRIX ALLOGRAFTSTransplantation, 1976
- The production of foot-and-mouth disease virus from BHK 21 C 13 cells grown on the surface of glass spheresBiotechnology & Bioengineering, 1976
- Cell Culture on Artificial Capillaries: An Approach to Tissue Growth in vitroScience, 1972
- Growth of Cell-strains and Primary Cells on Micro-carriers in Homogeneous CultureNature, 1967