Detection of complement fixation by enzyme linked immunosorbant assay (COMPELISA).

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Abstract
A method is described by which guinea pig complement fixation is detected with an enzyme linked immunosorbent assay (ELISA) technique. The method obviates the need for sensitized sheep red blood cells as an indicator of complement fixation and the titration of complement is not critical. The dose response curve has the advantage of being steep and the test result is read photometrically. As test serum and complement do not react together serum anticomplementary effects are eliminated. The ELISA complement fixation test was more sensitive than the conventional CFT for detecting antibodies to Brucella lipopolysaccharide.