THE SECRETORY ACTIVITY OF THE PANCREATIC DELTA CELLS OF THE YOUNG CHICK AND THE CHICK EMBRYO

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Abstract
It was attempted to follow the development of the secretory function of the pancreatic delta cell of the domestic fowl by an electron microscopic investigation. Rhode Island Red chicks on the 4th, 6th and 8th day after hatching and chick embryos on days 5–20 of incubation were used. The pancreas was obtained from the chicks after decapitation and was removed together with the digestive tube from living embryos. It was then pre-fixed in a 2·5% glutaraldehyde solution and post-fixed in 1% osmic acid buffered with phosphate, dehydrated with ethanol and embedded in epoxy resin. Ultrathin sections were cut with a Porter—Blum microtome and stained with lead citrate. An HU11A type electron microscope was used. The plate shows electron micrographs of delta cells of chick pancreas at different stages of development. At 11–14 days of incubation the delta cells showed marked secretory activity, although they could be seen before this stage

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