Cloning of an EcoRI fragment carrying E. coli tufA gene
- 1 January 1979
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 170 (2) , 231-234
- https://doi.org/10.1007/bf00337801
Abstract
EcoRI fragments of the transducing phage λfus3 DNA have been linked to the ColEl derivative plasmid RSF2124 (ColEl-Apr) DNA using bacteriophage T4 ligase. Among the plasmids formed, one designated pTUAl was found to contain the E. coli tufA gene. The proof for the presence of tufA gene in pTUAl is based on the following observations: (1) ability of pTUAl DNA and its EcoRI fragments to direct synthesis of EF-Tu in a cell-free protein synthesizing system; and (2) RNA·DNA hybridization of RNA transcribed from phage λrifd18 carrying tufB with DNA from pTUAl.Keywords
This publication has 28 references indexed in Scilit:
- The role of guanosine 5′-triphosphate in polypeptide chain elongationBiochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics, 1978
- A functional map of plasmid ColE1Molecular Genetics and Genomics, 1978
- A comparison of the activities of the products of the two genes for elongation factor TuMolecular Genetics and Genomics, 1978
- GENETICS OF BACTERIAL RIBOSOMESAnnual Review of Genetics, 1977
- Some Remarks on Recent Studies on the Assembly of RibosomesPublished by Elsevier ,1977
- Biosynthetic regulation of individual proteins in relA + and relA strains of Escherichia coli during amino acid starvationMolecular Genetics and Genomics, 1976
- Identification of two copies of the gene for the elongation factor EF–Tu in E. ColiNature, 1975
- The generation of a colE1-Apr cloning vehicle which allows detection of inserted DNAMolecular Genetics and Genomics, 1975
- Regulation of the in vivo synthesis of the polypeptide chain elongation factors in Escherichia coliBiochemistry, 1970
- A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membraneJournal of Molecular Biology, 1965