Regeneration in Alfalfa Tissue Culture
- 1 November 1985
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 79 (3) , 584-589
- https://doi.org/10.1104/pp.79.3.584
Abstract
The composition of phosphorus-containing compounds of intact lyophilized alfalfa tissue has been determined, in part, by solid-state 31P nuclear magnetic resonance. The tissue (Medicago sativa L., cv Regen S; and some of its crosses) was grown in culture under both nonregenerating and regenerating conditions, the latter enhanced by the addition of specific amino acids. Analysis of the 31P nuclear magnetic resonance spectra shows that regeneration is favored when metabolism occurs without the production of a low average intracellular pH.This publication has 14 references indexed in Scilit:
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