Genome expression analysis of Anopheles gambiae : Responses to injury, bacterial challenge, and malaria infection
Top Cited Papers
Open Access
- 19 June 2002
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 99 (13) , 8814-8819
- https://doi.org/10.1073/pnas.092274999
Abstract
The complex gene expression responses of Anopheles gambiae to microbial and malaria challenges, injury, and oxidative stress (in the mosquito and/or a cultured cell line) were surveyed by using cDNA microarrays constructed from an EST-clone collection. The expression profiles were broadly subdivided into induced and down-regulated gene clusters. Gram+ and Gram− bacteria and microbial elicitors up-regulated a diverse set of genes, many belonging to the immunity class, and the response to malaria partially overlapped with this response. Oxidative stress activated a distinctive set of genes, mainly implicated in oxidoreductive processes. Injury up- and down-regulated gene clusters also were distinctive, prominently implicating glycolysis-related genes and citric acid cycle/oxidative phosphorylation/redox-mitochondrial functions, respectively. Cross-comparison of in vivo and in vitro responses indicated the existence of tightly coregulated gene groups that may correspond to gene pathways.Keywords
This publication has 27 references indexed in Scilit:
- Drosophila innate immunity: an evolutionary perspectiveNature Immunology, 2002
- Substitution of the Thioredoxin System for Glutathione Reductase in Drosophila melanogasterScience, 2001
- A family of peptidoglycan recognition proteins in the fruit flyDrosophila melanogasterProceedings of the National Academy of Sciences, 2000
- A Lipopolysaccharide- and β-1,3-Glucan-binding Protein from Hemocytes of the Freshwater Crayfish Pacifastacus leniusculusJournal of Biological Chemistry, 2000
- Requirement for Croquemort in Phagocytosis of Apoptotic Cells in DrosophilaScience, 1999
- Phylogenetic Perspectives in Innate ImmunityScience, 1999
- Malaria infection of the mosquitoAnopheles gambiaeactivates immune-responsive genes during critical transition stages of the parasite life cycleThe EMBO Journal, 1998
- Innate immunity in higher insectsCurrent Opinion in Immunology, 1996
- cactus, a gene involved in dorsoventral pattern formation of Drosophila, is related to the IκB gene family of vertebratesCell, 1992
- In vitro induction of cecropin genes — an immune response in a Drosophila blood cell lineBiochemical and Biophysical Research Communications, 1992