The Use of Tris-HCl Buffer for Inoculation of Tomato Protoplasts with Tobacco Mosaic Virus
- 1 August 1976
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 32 (2) , 311-314
- https://doi.org/10.1099/0022-1317-32-2-311
Abstract
When tris-HCL was used as the buffer for inoculation of tomato protoplasts with tobacco mosaic virus, a greater proportion became infected than when phosphate was used. Using 0.05 M-tris-HCl buffer, pH 8.0, in the presence of poly-L-ornithine or poly- D-lysine, 50-80% infection was obtained.This publication has 0 references indexed in Scilit: