Characterization of an in vitro perfused rat ovary model: ovulation rate, oocyte maturation, steroidogenesis and influence of PMSG priming
- 1 May 1987
- journal article
- Published by Wiley in Acta Physiologica Scandinavica
- Vol. 130 (1) , 107-114
- https://doi.org/10.1111/j.1748-1716.1987.tb08117.x
Abstract
An in nitro perfused rat ovary model was characterized with respect to ovulation rate, oocyte maturation and steroidogenesis after priming with various doses of pregnant mare's serum gonadotrophin (PMSG) (10, 20 or 30 IU PMSG S.C. in the morning of day 28). In ovaries stimulated with LH 0.1 μg m1--1 after 1 h of perfusion the number of ovulations was significantly higher in the 20 IU PMSG group than in the 10 IU PMSG group (6.8±1.0 ovulations per treated ovary versus 2.4±1.2, P < 0.01). Priming with 30 IU PMSG did not result in significantly more or fewer ovulations than did 10 or 20 IU PMSG. No ovaries in the non-stimulated control groups ovulated. Oocytes retrieved directly at ovulation were mature (GVB or PB stage). Among oocytes retrieved from pre-ovulatory follicles punctured at 0, 2, 4, 6 and 8 h after stimulation with LH 0.1 μg ml-1 thtre was an increasing number of oocytes with GVB (0/10 at oh and 10/10 at 8 h). Stimulation with LH resulted in a rapid release of progesterone, testosterone and oestradiol into the medium, whereas non-stimulated control ovaries exhibited a slow rise of all steroids throughout the perfusion period. It is concluded that priming with 20 IU PMSG in this model results in an optimal number of ovulations and that this model provides a useful tool in studies of various kinds of modifying influences on the ovulatory process.Keywords
This publication has 25 references indexed in Scilit:
- Inhibition of Follicle-Stimulating Hormone-Induced Ovulation by Indomethacin in the Perfused Rat Ovary1Biology of Reproduction, 1987
- Ovulations in Rat Ovaries Perfused in Vitro with Follicle-stimulating Hormone1Biology of Reproduction, 1985
- Perfusion of the Rat Ovary In Vitro: Methodology, Induction of Ovulation, and Pattern of Steroidogenesis 1Biology of Reproduction, 1984
- Effect of Histamine and Histamine Blockers on the Ovulatory Process in the in Vitro Perfused Rabbit OvaryBiology of Reproduction, 1983
- The Study of Ovulation in the Isolated Perfused Rabbit Ovary. II. Photographic and Cinematographic ObservationsBiology of Reproduction, 1982
- The Study of Ovulation in the Isolated Perfused Rabbit Ovary. I. Methodology and Pattern of SteroidogenesisBiology of Reproduction, 1982
- Adsorption of steroid hormones by plastic tubingThe Journal of Steroid Biochemistry and Molecular Biology, 1981
- Changes in Follicular Fluid and Serum Concentrations of Steroids in PMS Treated Immature Rats following LH AdministrationBiology of Reproduction, 1979
- FSH and LH Release During the First Ovulation Period of Immature Rats Pretreated with PMSBiology of Reproduction, 1975
- The Role of Catecholamines and Nerves in OvulationBiology of Reproduction, 1974