Notizen: Quantification and Localisation of SH-Groups in Human Blood Serum Proteins

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Abstract
The thiol groups of human blood serum proteins were determined after 24 h interaction with dithionitrobenzoic-acid (DTNB) to an average of 538 .+-. 60 .mu.M serum. After treatment of the serum with [35S]DTNB, autoradiograms of the protein electrophoretograms revealed 2 main peaks. The first with 63% of total activity, in the albumin region, corresponded to 0.60 SH/mol. The second with 23% of total activity, in the .gamma.-globulin range, corresponded to 2.2 SH/mol. After 30 min incubation with DTNB, or with p-Ig[immunoglobulin]G only 0.2 SH/mol was found, the expected value already known from the literature. Autoradiograms taken from serum protein electrophoretograms after interaction with [14C]CMB showed only the main SH-peak in the albumin range. The SH-peak in the .gamma.-globulin region after 24 h incubation with [35S]DTNB was due to 1 highly labile S-S-bond which easily underwent disulfide exchange with DTNB.

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