Abstract
A chemically defined medium (CC-494) was used to study the lipid utilization of two flower spiroplasmas (Spiroplasma florkola and SR-3) and honeybee spiroplasma (AS-576). All three spiroplasmas reached comparable growth yields when lauric acid, myristic acid, or stearic acid replaced palmitic acid; palmitoleic acid, linoleic acid, or linolenic acid replaced oleic acid; and campesterol, ergosterol, β-sitosterol, or stigmasterol replaced cholesterol. None of the spiroplasmas grew when 5A-cholestane replaced cholesterol.

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