Expediting the Method Development and Quality Control of Reversed-Phase Liquid Chromatography Electrospray Ionization Mass Spectrometry for Pharmaceutical Analysis by Using an LC/MS Performance Test Mix
- 6 October 2000
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 72 (21) , 5211-5218
- https://doi.org/10.1021/ac000651d
Abstract
Mass spectrometry combined with liquid chromatography (LC/MS) has become an important analytical methodology in both pharmaceutical and biomolecule analyses. LC/MS, especially with reversed-phase HPLC (RP-LC), is extensively used in the separation and structural identification of pharmaceutical samples. However, many parameters have to be considered when a new LC/MS method is developed for either separation and structural analysis of unknown mixtures or quantitative analysis of a set of known compounds in an assay. The optimization of a new LC/MS method can be a time-consuming process. A novel kitLC/MS performance test mixcomposed of aspartame, cortisone, reserpine, and dioctyl phthalate has been developed to accelerate the process of establishing a new RP-LC/MS method. The LC/MS mix makes the evaluation and validation of an LC/MS method more efficient and easier. It also simplifies the quality control procedure for an LC/MS method in use.Keywords
This publication has 12 references indexed in Scilit:
- Quantitation and characterization of phospholipids in pharmaceutical formulations by liquid chromatography–mass spectrometryJournal of Chromatography A, 2000
- Quantification and Rapid Metabolite Identification in Drug Discovery Using API Time-of-Flight LC/MSAnalytical Chemistry, 2000
- Pharmacophore Fingerprinting. 2. Application to Primary Library DesignJournal of Chemical Information and Computer Sciences, 1999
- Impact of mass spectrometry on combinatorial chemistryJournal of Chromatography B: Biomedical Sciences and Applications, 1999
- A quality concept for impurities during drug development—use of the hyphenated LC–MS techniquePharmaceutical Science & Technology Today, 1998
- Dependence of ion intensity in electrospray mass spectrometry on the concentration of the analytes in the electrosprayed solutionAnalytical Chemistry, 1993
- Effect of the conductivity of the electrosprayed solution on the electrospray current. Factors determining analyte sensitivity in electrospray mass spectrometryAnalytical Chemistry, 1991
- Principles and practice of electrospray ionization—mass spectrometry for large polypeptides and proteinsMass Spectrometry Reviews, 1991
- Electrospray Ionization for Mass Spectrometry of Large BiomoleculesScience, 1989
- The determination of protein, oligonucleotide and peptide molecular weights by ion‐spray mass spectrometryRapid Communications in Mass Spectrometry, 1988