Site‐specific cleavage of double‐strand DNA by hydroperoxide of linoleic acid
- 9 July 1984
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 172 (2) , 231-234
- https://doi.org/10.1016/0014-5793(84)81131-x
Abstract
The breakage of double‐strand (ds) DNA by 13‐L‐hydroperoxy‐cis‐9, trans‐11‐octadecadienoic acid (LAHPO) was investigated by agarose gel electrophoresis of supercoiled pBR322 DNA and the site of cleavage on the DNA molecule was determined by the method of DNA sequence analysis using 3'‐end and 5'‐end‐labeled DNA fragments as substrates. LAHPO caused cleavage at the position of guanine nucleotide in dsDNA. LAHPO caused dsDNA breaks at specific sites, but linoleic acid (LA) and 13‐L‐hydoxy‐cis‐9,trans‐11‐octadecadienoic acid (LAHO) have no such effects on dsDNA. The active oxygen atom of the hydroperoxy group of LAHPO was perhaps responsible for the site‐specific cleavage of dsDNA.Keywords
This publication has 24 references indexed in Scilit:
- Dietary Carcinogens and AnticarcinogensScience, 1983
- Negative ames tests of epoxide fatty methyl esters derived from homolysis of linoleic acid hydroperoxidesFood and Chemical Toxicology, 1983
- Full structure of a mitomycin C dinucleoside phosphate adduct. Use of differential FT-IR spectroscopy in microscale structural studiesJournal of the American Chemical Society, 1983
- Identification of degradation products of d(C-G) by a 1,10-phenanthroline-copper ion complexJournal of the American Chemical Society, 1982
- The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primersGene, 1982
- Solubilization and properties of the enzyme-cleaving 13-l-hydroperoxylinolenic acid in tea leavesPhytochemistry, 1982
- Synthesis of the slow reacting substance of anaphylaxis leukotriene C-1 from arachidonic acidJournal of the American Chemical Society, 1980
- Lipid hydroperoxide reactivity with proteins and amino acids: a reviewJournal of Agricultural and Food Chemistry, 1979
- Lipoxygenase and Hydroperoxide Lyase in Germinating Watermelon SeedlingsPlant Physiology, 1976
- Free Radical Mechanisms of Lipid Damage and Consequences for Cellular MembranesPublished by Elsevier ,1976