The Amino Acid Sequence of the Large Plakalbumin Peptide and the C-Terminal Sequence of Ovalbumin
Open Access
- 1 January 1971
- journal article
- Published by CSIRO Publishing in Australian Journal of Biological Sciences
- Vol. 24 (3) , 525
- https://doi.org/10.1071/bi9710525
Abstract
The amino acid sequence of a 33-residue peptide isolated from plakalbumin by gel filtration in 6M urea at pH 3 and derived from the C-terminal portion of ovalbumin has been determined. Enzyme digestion of the hydrophobic areas by thermolysin, papain, ana subtilisin BPN' gave peptides with overlapping sequences. The peptides were fractionated by a combination of paper ionophoresis and chromatography and their sequences determined by the dansyl-Edman technique.Keywords
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