Cytoplasmic pH, a key determinant of growth factor‐induced DNA synthesis in quiescent fibroblasts
Open Access
- 7 October 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 190 (1) , 115-119
- https://doi.org/10.1016/0014-5793(85)80439-7
Abstract
In response to growth factors, quiescent fibroblast mutants lacking Na+/H+ exchange activity fail to elevate their cytoplasmic pH (pHi) and to reinitiate DNA synthesis at neutral and acidic pHo. A pHi threshold of ~7.2 exists, below which growth factors cannot set in motion the G0 to S phase transition. Restoration of the pHi defect in mutant cells restores the wild‐type phenotype. These findings, combined with the properties of another class of mutants able to grow at very low pHo, demonstrate that pHi modulated by growth factor activation of the Na+/H+ antiporter, plays a determinant role in growth control.Keywords
This publication has 26 references indexed in Scilit:
- A common sequence of calcium and pH signals in the mitogenic stimulation of eukaryotic cellsNature, 1985
- Phorbol ester and diacylglycerol mimic growth factors in raising cytoplasmic pHNature, 1984
- Serum, platelet-derived growth factor, vasopressin and phorbol esters increase intracellular pH in Swiss 3T3 cellsBiochemical and Biophysical Research Communications, 1983
- Analysis of growth factor “relaxation” in chinese hamster lung fibroblasts required for tumoral expressionJournal of Cellular Physiology, 1983
- Transport of H+ and of ionic weak acids and basesThe Journal of Membrane Biology, 1983
- Growth-Promoting Activity of Thrombin in Fibroblasts: Temporal Action and Early Biochemical Events in the G0 → G1 TransitionProceedings in Life Sciences, 1983
- Modifier role of internal H+ in activating the Na+–H+ exchanger in renal microvillus membrane vesiclesNature, 1982
- Growth factor activation of an amiloride-sensitive Na+/H+ exchange system in quiescent fibroblasts: coupling to ribosomal protein S6 phosphorylation.Proceedings of the National Academy of Sciences, 1982
- Stimulation of Na influx, Na-K pump activity and DNA synthesis in quiescent cultured cellsAdvances in Enzyme Regulation, 1981
- Intracellular pH and activation of sea urchin eggs after fertilisationNature, 1976