GLUCOSE AND LACTATE KINETICS IN GUINEA-PIGS FOLLOWING ESCHERICHIA-COLI ENDOTOXIN ADMINISTRATION

  • 1 January 1978
    • journal article
    • research article
    • Vol. 5  (1) , 11-21
Abstract
Glucose and lactate turnovers were evaluated during the early stages (first 3 h) of endotoxin-induced shock in unanesthetized male guinea pigs following the i.v. administration of 0.1 mg of E. coli endotoxin. Rate of appearance (Ra), rate of disappearance (Rd) and metabolic clearance rate (MCR) of glucose and lactate were determined by the primed-constant infusion of [6-3H]-glucose and NA L(+)[U-14C]-lactate. Arterial glucose concentration was moderately elevated, and the Ra and Rd of glocuse were increased significantly following endotoxin administration, while the slight increase in MCR was not statistically significant. Arterial lactate concentration was markedly increased and the Ra and Rd of lactate were significantly elevated. The percentage of [14C]-glucose derived from [14C]-lactate increased from 19% (control) to above 50% following endotoxin. No evidence of impaired peripheral extraction of glucose was found. The increased glucose Ra and the higher percentage of [14C]-glucose being derived from [14C]-lactate suggest increased gluconeogenesis during the early stages following endotoxin administration.