tRNAMetf2 gene in the leader region of the nusA operon in Escherichia coli.
- 1 January 1984
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 81 (2) , 409-413
- https://doi.org/10.1073/pnas.81.2.409
Abstract
The promoter-proximal portion of the operon containing the E. coli nusA gene was cloned. Its nucleotide sequence shows that genes for .**GRAPHIC**. and a 15-kilodalton protein of unknown function precede the nusA protein gene. The sequence suggests that the 3 genes form a single transcription unit. Consistent with this hypothesis, purified RNA polymerase formed full-length transcripts on the cloned DNA in vitro, although transcription was frequently arrested at the intercistronic site(s) between the gene for .**GRAPHIC**. and the 15-kilodalton protein.Keywords
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