Solid phase synthesis and characterization of two canine gut gastrin‐releasing peptides
- 1 August 1988
- journal article
- research article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 32 (2) , 141-152
- https://doi.org/10.1111/j.1399-3011.1988.tb00674.x
Abstract
Two canine gastrin-releasing peptides originally isolated from gut tissue extracts have been synthesized by solid phase methodology and purified by preparative reverse phase high performance liquid chromatography (RP-HPLC). The synthetic gastrin-releasing peptides GRP1-27 and GRP 5-27 were characterized with regard to homogeneity and composition using nine different RP-HPLC systems, mass spectroscopy, amino acid analysis, Edman degradation, methionine oxidation, and peptide mapping with tryptic, Staph aureus V8 protease and cyanogen bromide cleavage (the latter two systems performed only with GRP 1-27). Although a scarcity of the natural products prevented quantitative biological comparison of the synthetic and natural peptides, they were found to elute identically on RP-HPLC co-chromatography and similar dose dependent biological potencies were observed in canine antral muscle tissue contraction experiments. Indeed, all the peptides containing the bombesin-like carboxyl terminal decapeptide sequence studied to date have similar biological activities.Keywords
This publication has 26 references indexed in Scilit:
- Stimulation of gastrin release by bombesin and canine gastrin-releasing peptides. Studies with isolated canine G cells in primary culture.Journal of Clinical Investigation, 1987
- Studies on peptides. CXLV Synthesis of a 27-residue peptide amide corresponding to the entire amino acid sequence of canine gastrin-releasing polypeptide (cGRP).CHEMICAL & PHARMACEUTICAL BULLETIN, 1985
- Synthesis of A 27-residue peptide corresponding to the entire amino acid sequence of human gastrin-releasing peptide (hGRP).CHEMICAL & PHARMACEUTICAL BULLETIN, 1985
- Sequence analysis of a growth hormone releasing factor from a human pancreatic islet tumorBiochemistry, 1982
- Immunoreactive GRPPeptides, 1981
- Characterization of an avian gastric (proventricular) peptide having sequence homology with the porcine gastrin‐releasing peptide and the amphibian peptides bombesin and alytesinFEBS Letters, 1980
- Characterization of a gastrin releasing peptide from porcine non-antral gastric tissueBiochemical and Biophysical Research Communications, 1979
- Synthesis of a nonadecapeptide corresponding to residues 37-55 of ovine prolactin. Detection and isolation of the sulfonium form of methionine-containing peptidesJournal of the American Chemical Society, 1976
- Solid Phase Peptide Synthesis. I. The Synthesis of a TetrapeptideJournal of the American Chemical Society, 1963
- Derivate von L‐Methionin‐sulfoxyd und ihre Verwendung für PeptidsynthesenHelvetica Chimica Acta, 1961