Synthesis of cartilage-specific proteoglycan by suspension cultures of adult chondrocytes

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Abstract
Chondrocytes isolated form larynges of adult pigs were cultured as cell suspensions for at least 4 days before use. During continuous-labeling experiments in nutrient medium for 18 h with 35SO42- and [3H]glucosamine as precursors, some macromolecular polyanionic material was synthesized which behaved on gel chromatography as proteoglycan. Gel chromatography on Sepharose 2B showed that a proportion of the proteoglycans in the medium appeared to be aggregated, and was dissociated in 4M-guanidinium chloride. The dissociated proteoglycan interacted with hyaluronic acid. Newly synthesized proteoglycan was larger than the average total cetylpyridinium chloride-precipitable material assayed as uronic acid alone.